2007 Annual Report 1a.Objectives (from AD-416) To identify and quantify vitamin K and related compounds in representative samples of foods and dietary supplements to improve and expand analyses to the USDA Nutrient Databases. 1b.Approach (from AD-416) The Nutrient Data Laboratory (NDL) is responsible for developing and disseminating authoritative composition database on foods and dietary supplements in the U.S. diet. NDL has developed a Key Foods approach to help establish priorities for foods to be analyzed. Key Foods are those identified as contributing up to 75% of any one nutrient of public health significance. Key Foods from the core of foods to be analyzed supplemented by other foods deemed of particular interest due to their frequent use as ingredients or content of nutrients of emerging interest. Sampling plans will be developed for each type of food sampled, to assure a representative sampling of the food supply. Dietary supplements for analysis are identified using data from NHANES and market share data from industry sources. Samples of foods or dietary supplements will be delivered to the cooperator for analysis. In a limited number of cases the cooperator may need to develop matrix specific methods for handling and analyzing food or dietary supplement samples and preparing aliquots for shipment to other researchers for analysis of additional components. The cooperator will also offer expertise in interpretation of results of the analyses of vitamin K and related components. 3.Progress Report This report documents research conducted under a Specific Cooperative Agreement between ARS and Tufts University. Additional details of research can be found in the report for the in-house associated project 1235-52000-051-00D, "Development of Accurate and Representative Food Composition Data for the U.S. Food Supply." During 2007, 52 food and ingredient samples, representing 10 food items, were analyzed for two forms of vitamin K, phylloquinone, the primary dietary source of vitamin K: in the U.S., and its hydrogenated form, dihydrophylloquinone (dK). In addition, menaquinone, found only in animal products, has also been analyzed. NDL staff continued to add new vitamin K values to SR, as well as update existing data. Of the 119 new values added to SR, 39 were analytical values from this project. Of the 299 updated values, 63 were analytical. This project was monitored through the periodic receipt of reports of analytical results, e-mail, and phone calls. Results were reviewed by NDL's Quality Control Committee and any questions referred to the principal investigator for resolution.