%PDF-1.6
%
198 0 obj
<>
endobj
197 0 obj
<>stream
Acrobat Distiller 5.0 (Windows)
Shiftwork, Cortisol, Melatonin, Core Body Temperature, Circadian Rhythms, Clockwise, Counter-Clockwise
2002-11-26T14:17:18Z
2011-11-22T12:36:13-06:00
2011-11-22T12:36:13-06:00
PageMaker 7.0
application/pdf
Boquet A, Cruz C, Nesthus TE, Detwiler C, Knecht W, and Holcomb K
A laboratory comparison of clockwise and counter-clockwise rapidly rotating shift schedule, Part III: Effects on core body temperatures and neuroendocrine measures
uuid:60c475b8-c785-4df7-b0cc-23a68ef391f3
uuid:8bf0a7ff-6cce-465e-9468-dd872686b4d1
endstream
endobj
63 0 obj
<>
endobj
9 0 obj
<>
endobj
64 0 obj
<>
endobj
139 0 obj
<>
endobj
135 0 obj
<>
endobj
141 0 obj
<>
endobj
144 0 obj
<>
endobj
149 0 obj
<>
endobj
150 0 obj
<>stream
endstream
endobj
151 0 obj
<>
endobj
145 0 obj
<>stream
BT
/F1 1 Tf
10 0 0 10 309.36 45 Tm
0 0 0 1 k
/GS14 gs
0.023 Tc
0 Tw
(B1)Tj
/F2 1 Tf
14.3 0 0 13 272.28 703.6801 Tm
0.012 Tc
-0.013 Tw
[(APP)12(ENDIX B)]TJ
11 0 0 11 216.96 689.64 Tm
0.023 Tc
-0.0091 Tw
(Cortisol and Melatonin Assay Procedures)Tj
-13.1782 -2.5418 TD
0.0015 Tw
(A. Assay for Melatonin)Tj
/F1 1 Tf
0 -1.2764 TD
0.0234 Tc
0.0942 Tw
[(1.)-380.2(Incubation buffer was distributed as described into each of the individual polystyrene tubes. The NSB)]TJ
1.1782 -1.2764 TD
-0.0662 Tw
(\(blank\) tubes received 500 L, and the MB \(maximum binding\) tubes received 400 L. The standard tubes,)Tj
0 -1.2654 TD
0.0232 Tc
-0.0409 Tw
(labeled as A through F, received 400 L of the corresponding standard solution. The samples \(400 L\) and)Tj
0 -1.2764 TD
0.0234 Tc
-0.0035 Tw
(the controls \(400 L\) were distributed to their corresponding tubes.)Tj
-1.1782 -1.6036 TD
-0.0006 Tw
[(2.)-380.3(100 L of the melatonin antibody was delivered to all except the NSB and T \(total activity\) tubes.)]TJ
0 -1.5927 TD
0.0239 Tc
-0.049 Tw
[(3.)-379.7(100 L of the )]TJ
6.4 0 0 6.4 147.6 574.08 Tm
0.0188 Tc
0 Tw
(125)Tj
11 0 0 11 157.68 570.48 Tm
0.023 Tc
-0.0491 Tw
(I-melatonin tracer was added to all tubes, and all were vortexed. The T tubes were set aside)Tj
-6.6109 -1.2764 TD
0.0235 Tc
-0.0038 Tw
(at this point, since they required no further processing until the final step.)Tj
-1.1782 -1.6036 TD
0.0233 Tc
-0.0029 Tw
[(4.)-380.3(All tubes were incubated for 20 hours at 4C in the refrigerator.)]TJ
0 -1.5927 TD
-0.0876 Tw
[(5.)-380.2(100 L of the second antibody suspension, continuously being stirred during this step, was added to all assay)]TJ
1.1782 -1.2764 TD
-0.0039 Tw
(tubes \(except T tubes\). The assay tubes were maintained at 4C during this process.)Tj
-1.1782 -1.6036 TD
0.0316 Tw
[(6.)-380.3(The tubes, with the added second antibody, were incubated for 15 minutes at 4C. Then, 1 mL of cold,)]TJ
1.1782 -1.2655 TD
-0.0021 Tw
(distilled water was added to all the tubes \(except the T tubes\).)Tj
-1.1782 -1.6036 TD
0.1241 Tw
[(7.)-380.2(The tubes were centrifuged for 2 minutes at 2000 x g and 4C. The supernatants were aspirated and)]TJ
1.1782 -1.2764 TD
-0.0066 Tw
(discarded \(except the T tubes\), and the precipitates were retained for counting.)Tj
-1.1782 -1.5927 TD
-0.0033 Tw
[(8.)-380.3(Finally, the tubes were subjected to counting for 2 minutes each in the Gamma 5500 counter.)]TJ
/F2 1 Tf
0 -2.88 TD
0.0015 Tw
(B. Assay for Cortisol)Tj
/F1 1 Tf
0 -1.2655 TD
0.0227 Tw
[(1.)-380.4(Each standard, A through F, was diluted 1-in-10 with double-distilled water. The standard tubes, coated)]TJ
1.1782 -1.2764 TD
-0.0096 Tw
(with antibody and labeled A through F, received 200 L of the corresponding standard. The NSB \(blank\))Tj
T*
-0.0009 Tw
(tube was uncoated and received 200 L of the A standard.)Tj
-1.1782 -1.5927 TD
-0.0159 Tw
[(2.)-380.2(200 L of each saliva sample was pipetted into antibody coated tubes. This was done in duplicate for each)]TJ
1.1782 -1.2764 TD
0.0216 Tc
0 Tw
(sample.)Tj
-1.1782 -1.6036 TD
0.0235 Tc
-0.0093 Tw
[(3.)-380.1(1.0 mL of )]TJ
6.4 0 0 6.4 132.6 307.32 Tm
0.0188 Tc
0 Tw
(125)Tj
11 0 0 11 142.56 303.6 Tm
0.0234 Tc
-0.0101 Tw
(I-cortisol was pipetted into every tube and each tube was vortexed for mixing. Two uncoated)Tj
-5.2364 -1.2655 TD
0.0231 Tc
0.0039 Tw
(tubes, labeled T, received 1.0 mL of )Tj
6.4 0 0 6.4 250.08 293.28 Tm
0.0187 Tc
0 Tw
(125)Tj
11 0 0 11 260.16 289.68 Tm
0.0235 Tc
0.0046 Tw
(I-cortisol for obtaining a total radioactive count.)Tj
-17.1055 -1.6036 TD
0.0233 Tc
-0.0275 Tw
[(4.)-380.3(The tubes were incubated for 3 hours. After incubation, all tubes, except T tubes, were decanted for 2 to 3)]TJ
1.1782 -1.2764 TD
0.0222 Tc
0 Tw
(minutes.)Tj
-1.1782 -1.5927 TD
0.0233 Tc
-0.0042 Tw
[(5.)-380.3(All tubes were then counted for 1 minute using the Beckman Gamma 5500)]TJ
6.4 0 0 6.4 423.48 244.08 Tm
0.0217 Tc
0 Tw
(TM)Tj
11 0 0 11 433.8 240.48 Tm
0.0233 Tc
-0.0041 Tw
( counter.)Tj
ET
endstream
endobj
146 0 obj
<>/Font<>/ProcSet[/PDF/Text]>>
endobj
4 0 obj
<>
endobj
5 0 obj
<>
endobj
166 0 obj
<>
endobj
167 0 obj
<>stream
HyPI2Q#%*àrH8DDp @Dp0$
PDdgvBEtYXuf*wuWmUW]OW_f#H7Ե¬%ޚ0/_b\eɐk6êcH@anI~z>wM\gOOGCtY<0Y*) 9)\a>_$H]geqw̳A~a?M/|~ _;K 43±Iz6fƑƃd/9\B12yBfaZ 6B{X$f/tXWӧf-")*#Ϟ֮Wt7+soܪUV^=gm{.}5>Pp*G/[k=מ\4 b?R]7606qwN?HP{|Lxبx{M7naH|^zu?sI@@8@@E`K%h$pQ-$8%T,jغ{p|ǵNMؘ؆]]coݍIxɗ5IST``|oQ>}dCfG(;"'Ry]/+:[ܿ% %RyⲎn_+w.?#qK*嵊\e*#Zacg
ee,]":"?R̾Ⱥ9&Ã_%
ds'`|,1g3ՙҺN5fvɍ:t:> n8S_lO\f*';P:K8ٙ20Jr';.[I
e^"B~MGa1#Zȯ/Rtɮ횁Rw(EqWaKs[㵔*4_]{f4ü*